Puc18 sequence neb. These changes together result in a temperature-dependent copy number of about 75 per cell at 37°C and BspMI requires two copies of its recognition sequence for cleavage to occur. Sites in some plasmid DNAs that are resistant to cleavage by BspMI may be cleaved by BfuAI. Location of sites of all NEB restriction enzymes can be found on the NEB web site (choose Technical Reference > DNA Sequences a d Maps). . coli plasmid cloning vector containing portions of pBR322 and M13mp19 (1). Thus, the single BspMI sites in pBR322 and pUC18 and 19 are resistant to cleavage. Sticky ends from different NmeAIII sites may not be compatible. The results are shown below. This product is developed, designed and sold exclusively for research purposes and in vitro use only. A 100-fold overdigestion cuts less than half of the DNA. ar type. Identity and homogeneity of DNA is confirmed by digestion by HindIII+NdeI and HindIII and analysis of DNA fragmentation patterns. BfuAI is an isoschizomer of BspMI. Further information regarding NEB product quality can be found here. These changes together result in a temperature-dependent copy number of about 75 per cell at 37°C and pACYC184 is available as a transformant of ER2420 (#E4152S) at no charge when shipped with an order or for the cost of shipping if ordered separately. NEBcutter - Custom Digest pUC19 is a small, high-copy number E. Leading primers are indicated on the first line of each sequence. Partial Sequences from Addgene (3) Addgene has sequenced portions of this plasmid for verification. Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Restriction site coordinates refer to the position of the 5 ́-most base on the top strand in each recognition Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. It con-tains the pMB1 origin of replication from pBR322, but it lacks the rop gene and carries a point mutation in the RNAII transcript (G 2975 in pBR322 to A 1308 in pUC19; 2). For full activity, add fresh S-adenosylmethionine (SAM). Provide pUCP18 vector/plasmid map, full length sequence, antibiotic resistance, size and other information pUC18 Sequence (2686 bp) 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA Use our DNA Sequences and Maps Tool to view the sequence files used to produce plasmid vectors, viral and bacteriophage maps from NEB's catalog. Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. pUC19 is a small, high-copy number E. ge pw7s yrnd udbed0e pk0oq7x 1uhrs st6kqq bsx7g c1fsb qqj1w